Business Plan-Development of an online fitness service Essay

Marketable strategy Development of an online wellness administration - Essay Example A greater level of their own commitment is put resources into leading statistical surveying. A compelling statistical surveying illuminates the business, it is through these investigates that the speculators distinguish practical business openings and the most ideal methods of working the offices (ALBRECHT, 2011). They be that as it may, pick not to incorporate the sum utilized in leading the statistical surveying since the sum isn't legitimately put resources into the business. Its commitment to the benefit of the business is along these lines in a roundabout way identified with the real activities of the business. The ten thousand pounds is utilized to make sure about a business premise. This is an online exercise center and don't accordingly require a major genuine space. Notwithstanding this comprehension, the business despite everything requires a reason where the real activities will occur. The speculators will require a space to set up their PCs and a real rec center in which they will create their preparation instructional exercises. Online rec center suggests that the business puts resources into distributing exercise instructional exercises over the web. In this, the business requires the creation of high caliber, successful and opportune exercise units that in this manner offer importance to the genuine space for which the speculators allot 5,000 pounds. They buy the space, which makes the structure a fixed resource for the speculators. The speculators distribute an extra 4,000 pounds in the acquisition of hardware. This incorporates the rec center offices and PCs. A portion of this hardware in the long run mileage down however a lion's share of these, for example, the extremely significant PCs and the preparation offices. The hardware will perhaps be utilized for in excess of a solitary money related year; this thusly makes it inhumane toward spot such resources as current. Notwithstanding, the ease of use of these offices depend intensely on the security system that the financial specialists sets up for their business. In the event of a

Criminal Law Essay

Criminal Law Essay Criminal Law Essay CRIMINAL LAW STUDY GUIDE Family unit VICTIMIZATION URBAN PEOPLE FACE HIGHER RISK THAN RURAL PEOPLE, HIGHER HOUSEHOLD INCOME ASSOCIATED WITH HIGHER VICTIMIZATION RATE, HIGHR RATES IN HOUSEHOLDS WITH MORE PEOPLE, SEMI-DETACHED,ROW, AND DUPLEX HOMES FACE THE HIGHEST RATE AND RENTERS FACE HIGHER RATES THAN OWNERS People WHO LIVED IN THEIR HOMES FOR ONLY A SHORT PERIOD OF TIME ARE MORE AT RISK OF VICTIMIZATION MOST VICTIMS LOST UNDER $500 AND MOST ITEMS WERE NEVER RECOVERED Just 3 IN 4 CASES WERE INVESTIGATED BY THE POLICE AND VICTIMS WERE MORE LIKELY TO PARTICIPATE IN VICTIM OFFENDER MEDIATION PROGRAM. Wrongdoing Wrongdoing IS A DETERMINATION OF GUILT IN A CRIMINAL COURT ALSO REFERED TO AS THE â€Å"BLACK LETTER†, IT’S THE VIOLATION OF A CRIMINAL LAW, VIOLATION OF SOCIAL NORMS. THE ESSENTIAL CHARACTERISTIC OF CRIME IS THAT Behavior WICH IS PROHIBITD BY THE STATE AS AN INJURY TO THE STATE. Native JUSTICE CRIMINAL CASES IN CANADA GANNON AND MOLHOREAN Feelings Illegitimate ACT Chargeable BECARRIA TASERS TASERS STANTS FOR â€Å" THOMAS A. Quick ELECTRIC RIFFLE† HAND HELD WEAPON THAT DELIVERS A JOLT OF ELECTRICITY FROM A DISTANCE INMOBILIZES INDIVIDUALS MUSCLES Exceptionally EFFECTIVE UCR Separation Separation IF THE DIFFERENTIAL TREATMENT BASED ON NEGATIVE JUDGMENTS RELATED TO GROUP MEMBERSHIP. Fundamental DISCRIMINATION-DISCRIMINATION IN ALL ASPECTS OF THE OPERATION OF THE CJS INSTITUTIONAL DISCRIMINATION-DISPITES IN THE OUTCOMES OF POLICIES Logical

Symphony 9, Op. 95 From the New Blog

Symphony 9, Op. 95 “From the New Blog” Reading a seven-month-old issue of People, I discovered this week that Gwen Stefani, in addition to designing a bizarre new line of clothing, has now taken to paying an entourage of four Japanese girls to follow her around as a living fashion accessory. Her inspiration in this endeavor was apparently the girls who hang out in the Harajuku shopping district of Japan and wear funky punk-rocker clothing. It occurred to me that when I am wealthy and powerful (no doubt as a result of my MIT education), rather than buying a huge house or a penthouse apartment or taking lavish and exotic vacations, Im just going to use my fortune to pay people to follow me around and make me look cooler. Id like to dedicate this entry to the 44 people who checked out my blog yesterday even though I havent updated in 6 days; you guys are my American Idols. I also discovered while reading Guns, Germs, and Steel that the QWERTY keyboard that Western Civilization knows and loves so well was actually designed to reduce typing speed. It turns out that on the first typewriters ever invented had a tendency to jam up if adjacent keys were struck rapidly in succession. So, the QWERTY keyboard was created with two goals in mind: a) minimize the number of adjacent keys struck while typing, therefore requiring as much separation of the hands as possible b) slow typing speed to prevent and key breakage at all. To accomplish the latter, designers placed the most common letters on the left of the keyboard, ensuring that right-handed typists would be forced to rely on their weaker hand as much as possible. In the 1930s, when typewriter were more durable, making these initial design specifications obsolete, the typewriter/secretary industry (Big Typewriter) was already powerful enough to resist any change in keyboard layout, even though studies with improved layouts, such as the Dvorak keyboard, showed that the QWERTY keyboard was 95 percent less efficient. So, I decided that itd be a good idea to learn how to use the Dvorak keyboard and thereby increase my efficency 20 times. Plus, I thought I could put it on my resume and then have an amusing anecdote to tell when asked about it at interview. Unfortunately, according to Froogle, Dvorak keyboards normally run in the range of $60, and even a set of overlaying stickers, to convert a normal keyboard to Dvorak, would set me back about $20. Thats just stupid. So, Im hoping that Ill either be able to find one abandoned in a basement when I get back to MIT or that Ben will buy me one to advance the cause of my blogging. Wish me luck! Ill be back at MIT tomorrow night to organize my train-wreck-war-zone-hit-by-a-tornado mess of a room. Oh, and I shudder to think at how fast Mitra would type on a Dvorak.

Differences Between Carbon-12 and Carbon-14

Carbon-12 and carbon-14 are two isotopes of the element carbon. The difference between carbon-12 and carbon-14 is the number of neutrons in each of their atoms. This is how this works. The number given after the atom name indicates the number of protons plus neutrons in an atom or ion. Atoms of both isotopes of carbon contain 6 protons. Atoms of carbon-12 have 6 neutrons, while atoms of carbon-14 contain 8 neutrons. A  neutral atom would have the same number of protons and electrons, so a neutral atom of carbon-12 or carbon-14 would have 6 electrons. Although neutrons do not carry an electrical charge, they have a  mass comparable to that of protons, so different isotopes have different atomic weight. Carbon-12 is lighter than carbon-14. Carbon  Isotopes and Radioactivity Because of the different number of neutrons, carbon-12 and carbon-14 differ in terms of radioactivity. Carbon-12 is a stable isotope; carbon-14, on the other hand, undergoes radioactive decay: 146C → 147N 0-1e (half-life is 5720 years) Other Common Isotopes of Carbon The other common isotope of carbon is carbon-13. Carbon-13 has 6 protons, just like other carbon isotope, but it has 7 neutrons. It is not radioactive. Although 15 isotopes of carbon are known, the natural form of the element consists of a mixture of only three of them: carbon-12, carbon-13, and carbon-14. Most of the atoms are carbon-12. Measuring the difference in the ratio between carbon-12 and carbon-14 is useful for dating the age of organic matter  since a living organism is exchanging carbon and maintaining a certain ratio of isotopes. In a diseased organism, there is no exchange of carbon, but the carbon-14 that is present undergoes radioactive decay, so over time the change in isotope ratio becomes greater and greater.

Generic and Value Adding Strategies of Christian Dior and Balenciaga Free Essay Example, 2500 words

Strategies of firms are considered to lean towards the strategic scope focusing on the demand, and the strategic strength concentrating on the supply. Porter (1980) further simplified the method by reducing the strategy to three specific dimensions, which included: cost leadership, differentiation, and segmentation. These aspects are also present in the companies discussed in the previous segment. From these general strategies, specific schemes of operations are designed. Since Christian Dior and Balenciaga are in the same industry, it is important to analyze both companies approaches. Focus on a single product design is the key to efficiency. This is the usual method used by Balenciaga to create its products. Most of the employees of the company are from within the group, and the lean but mean workforce structure is evident in the Balenciaga s desire to be efficient. The efficiency of the house is observed in most of its stores. The spacious area houses most of the products arrange d with efficiency. Store managers are making constant evaluation especially with the performance of the employees. The company continues to aim at lessening its costs of operations without sacrificing quality. The process of differentiation is guided by the skills that are enhanced by the designers of the company. We will write a custom essay sample on Generic and Value Adding Strategies of Christian Dior and Balenciaga or any topic specifically for you Only $17.96 $11.86/pageorder now In addition, the distribution channels of the company are strong with the help of Gucci and other subsidiaries within the group. Basically, the company focuses on the quality of the products and its appeal to the consumers. In addition, the focus strategy of the company has been celebrities and other well-known individuals. Christian Dior values efficiency more than the other attributes that the company promotes. The fashion group has the most number of employees and it provides better manpower in the production. In addition, delegation tasks are simpler because of the vast workforce expertises.

Variation Of Acoustics Within A Space Architecture Essay Free Essays

Concrete would bring forth loud resounding sound whereas rug would absorb sound. A little Dark and loud infinite would arouse tenseness in the user where as a infinite meant to portray joy would be big, good lit and have proper acoustic values. Aural architecture plays many important functions in assorted types of infinites such as musical, societal, navigational, aesthetic and symbolic. We will write a custom essay sample on Variation Of Acoustics Within A Space Architecture Essay or any similar topic only for you Order Now In a musical infinite, the echo blends the note sequences into chords. In a societal infinite it changes the distance between people in eating houses. In a navigational infinite it permits traveling through a dark infinite by echo sounding, as an aesthetic infinite it provides a delighting aural texture and as a symbolic infinite it can be associated with spiritual significance in the context of a infinite such as a cathedral ( Blesser 2009 ) The image above shows the shrine of St. Werburg in the Chester Cathedral. This shrine has six deferrals for kneeling pilgrims to put their caputs while praying. This pit amplifies and filters the sound giving the individuals voice a dramatic and emotional strength. The reverberations give the sense of being in another universe, the elaboration gives the sense of familiarity and the ocular isolation contributes to privateness. Through a insistent use for rites and ceremonials, the ocular and aural belongingss of such objects or geometries can get a symbolic significance. In the ocular universe, these would be called icons, and the equivalent of it in the aural universe would be known as an earcon ( Blesser and Salter,2007 ) . The user will be cognizant of the enormousness, geometry and stuff of the cathedral by the feeling of the reverberation and re-echo within the infinite. If the stuff used for the cathedral was carpet alternatively of rock, it would be acoustically softened every bit good as the spacial and acoustic dimensions of the architecture will be lost. ( Holl, Pallasmaa and Gomez, 2006 ) Aural architectures affect on societal infinites is clearly apparent in figure 2 shown below. When we think of architecture we tend to visualise the belongingss of the infinite that can be seen, specially the boundaries that are restricting us to a limited infinite and curtailing our motion every bit good as our legal rights of entree. On the other manus, aural boundaries are different to ocular boundaries as sound flows through every little gap and cranny. Fig 2. Hogarth ‘s angered instrumentalists. Hogarth portrays the discouragement of a instrumentalist who finds that his private music room and the uproar of the street are in fact a singleA aural infinite. The figure above demonstrates how the sounds from the street flow freely through the unfastened window of the house. Hence the street and the room with the unfastened window portion the same aural infinite. Fig 3. Dan Kish ‘s Team Bat taking unsighted adolescents on a bike drive The function of aural architecture in navigational infinites is barely of all time recognized except by the unsighted persons who rely on echo sounding to assist them travel around ( fig 3 ) . We are non usually cognizant that we posses the ability to hear spacial properties such as an unfastened window or a wall. However every person has an developing ability to utilize their ears to supplement vision. Potholers can acquire a sense of the countries of the cave that are wholly hidden by listening to the echo and reverberations ( Blesser and Salter,2007 ) . Anyone who has been spellbound by the sound of H2O beads in the darkness of a ruin is a informant to the extraordinary capacity of the ear to carve our volume into the nothingness of darkness ( Holl, Pallasmaa and Gomez, 2006 ) . The acoustic properties of a musical infinite have a important consequence on the temper and behaviour of an person. This consequence is best demonstrated in high impact infinites instead than ordinary infinites. Stockhausen ‘s public presentation of â€Å" Stimmung † in the Jaita caves near Beirut proves this. The public presentation was on a platform constructed over the abbeys and the audience was 80 thousand off from the performing artists, unlike in normal concert halls. This allowed the natural acoustics to rule the direct sound. After listening to the public presentation, a Catholic priest said it was one of the longest and happiest supplications he had of all time known. Fig 4. Stockhausen ‘s public presentation of â€Å" Stimmung † in the Jeita Cave near Beirut. The acoustic belongingss of a high impact infinite have a greater and more effectual impact on the temper and emotion of the user than in an ordinary infinite. However one can all remember the acoustic abrasiveness of an inhibited and unfurnished house as apposed to the affableness of a lived place. Juhani Pallasmaa writes, ‘ every edifice or infinite has its characteristic sounds of familiarity or monumentality, invitation or rejecting, cordial reception or ill will. ‘ The map of aural architecture in an aesthetic infinite is similar to adding decorations and texture to our ocular infinites to do them look alone and interesting. In the same mode we can add aural embroideries to alter the acoustics of the environment every bit good. In an experiment done by Eusebio Sempere, where he made a sculpture with unstained steel tubings that rotate at its base. It acts as a sonic filter which filters out passage of certain frequences, therefore the hearer on one side heard a tonic alteration of the sound geting from the other side. This sculpture is a ocular equivalent of the colored glass prism. Fig 5. Eusebio Sempere ‘s sculpture inA Madrid has alone acoustic belongingss. Architecture is best perceived every bit sound as it leaves a batch to the imaginativeness of the user and it is said that imaginativeness is needed to do sense of perceptual experience. Even though sound is non the dominant medium for comprehending architecture, it is successful in giving individuality to architecture as sound creates an atmosphere beyond map. In his book Eyes of the Skin, Pallasmaa states that ‘Modern architecture at big has housed the mind and the oculus, but it has left the organic structure and the other senses, every bit good as our memories, imaginativeness and dreams, homeless ‘ ( pg 19 ) . â€Å" You see things and state why? But I dream things that ne’er were and state why non? † Unknown Imagination is the ability to organize a mental image of something that is non straight perceived through the senses. It is the ability of the head to organize mental scenes, objects or events that do non be, are non present or have non happened ( Remez Sasson ) . Imagination is non merely limited to seeing images in the head. It includes all the five senses and feelings. One can conceive of a sound, gustatory sensation, odor, a physical esthesis a feeling or emotion. It is imagination that makes it possible to see a whole universe inside the head. It gives us the ability to look at any state of affairs from a different point of position. It has a really of import function and value in an person ‘s life and is non simply merely woolgathering. Every person possesses some ability to conceive of, but in some it may be developed more than others. We all use it, consciously or unconsciously, in our day-to-day life whether it is to read, compose, be after a party or a trip, to depict an event, to state or narrative, or merely merely giving waies to acquire to a specific finish. The originative power of imaginativeness is needed to accomplish success in any field, and some persons have a greater imaginativeness than others. It is a power beyond originative visual image, positive thought and avowals ( Remez Sasson ) . Basically, imaginativeness is the power of doing something up in one ‘s caput. Perception depends on the world-wide position of a individual and imaginativeness is needed to do sense of these perceptual experiences. Make you prefer your ain dreams or person else ‘s? How about your ain phantasies or person else ‘s? ( Anthony Megna ) . This is the really ground why many persons prefer reading a book in comparing to watching a film, as the film is person else ‘s position and non their ain. The spectator has no power to conceive of the characters, topographic points or the events of the narrative as everything can be seen. Alternatively, when reading a novel, the reader has the chance to put out their ain readings of the narrative and has the power to conceive of the characters, topographic points and incidents in the narrative as he/she wants. Hence reading a book leaves one with a greater capacity to conceive of than watching a film, which is person else ‘s fiction. â€Å" Whenever I start reading a book I ever come up with how I think a character should look or sound, so when versions come out and there is eventually a face and voice to the character and it is different t han the 1 in my caput, it can be hard for me to accept † says Lauren, a Harry Potter fan. By and large when comparing a film to a book, the book wins the lucifer because with a book, one can acquire more information, more scenes and a better How to cite Variation Of Acoustics Within A Space Architecture Essay, Essay examples

Role Of Creating Sustainable Organizations â€Myassignmenthelp.Com

Question: Discuss About The Role Of HR In Creating Sustainable Organizations? Answer: Introduction Managing Human Resources includes performing the functions related to the human resources of the organisation which is to be taken care by the Human Resources manager. It includes functions like planning and allocating resources, training and development activities, motivating and encouraging employees, managing the demand and supply of employees in the organisation and many more (Guest, 2011). The report is based on the case study where two hospitals are mentioned. One has a separate HR department while other does not have an HR department and the functions of HR are handled by the managers themselves along with CEO. It will be recommended that what actions should be taken by the CEO of the hospital which does not have separate HR department so that the operational improvement can be made in the hospital. The report further discusses building capabilities of sustainable HR in the organisations. It will help the hospital in developing the sustainable HR practices Recommended course of action to the CEO in the first hospital to improve the operation of HRM As per the case study, the first hospital does not have any dedicated HR department and the CEO himself with the support of finance manager runs the functions of an HR which involves recruitment and selection, training development and other functions. Because of no specific responsibility assigned, the hospital is facing challenges of disputes, staff grievances, absenteeism and more. It is recommended to the first hospital that proper Human Resource department needs to be settled to manage the growing concerns and issues among the staff and management. It is because the Human resource department focuses specifically on planning the management of Human resources of the organisation for their betterment and development in the future. It also aims at making the employees committed to the organisation and providing their full potential in the hospital so that the users feel satisfied and prefer the services again in future. According to Bloom and Van Reenen (2011), Human resource management is the management of the human capital of the company. It aims at maximising the performance of the employees in order to meet the strategic objectives of the company (Schroeder, 2012). The first hospital needs a separate human resource department in order to improve the operations of HRM. It will plan the right mix of manpower in the hospital so that the services, their volume and quality can be maintained in the hospital. It will also plan about training the individuals to make them more competent and to develop positive attitude in them for the hospital. This will let the CEO and other managers to focus on their core operations. Also, it is not the job of the middle managers to handle the functions of HR but they should have an option to access the HR department in case of any dissatisfaction from the management. This satisfies the employees because they know they will be taken care of in the hospital. A clean and professional working environment will create positive attitude in the employees and this will ultimately improve their performance (Tiwari Saxena, 2012). The HR department of the hospital will focus on manpower planning, recruitment and selection, training and development, Health care of employees and working environment of the hospital and future growth and development of employees (Sendogdu, Kocabacak Guven, 2013). The development of HR policies and practices will make the procedures transparent which will make the hospital capable of meeting the challenges related to human resources which includes the staff problems, disputes and increased absenteeism which ultimately affects the quality of care and increases the accidents in the hospital which includes the staff and patients. The implementation of HR policies by a separate HR department will definitely improve the HR operations and overall business of the hospital. Key features of building a sustainable HR capability In the opinion of Kramar (2014), there is a strong relationship between sustainability and Human Resource Management. HR is not just about the expansion of personnel management but also the organisational development for enhancing the effectiveness of the same. The organisational capabilities can be explained as the factors which drives the results for the organisation. Every company have their own capabilities. If the company has the ability of managing its people, it gives competitive advantage to the organisation and these capabilities are gained through the people and their collective efforts. A sustainable HR capability involves the following features: Increased focus on sustainability: For building the sustainable HR capabilities, the focus has to be given on sustainability. The employees need to be made understand about the sustainable business practices and how they can create long term value for the human resources as well as for the organisation (Stankeviciute Savaneviciene, 2013) Meeting the expectations of the employees: If the expectations of the human resources of hospital is met effectively, it will ensure the positively in the employees for the hospital and it will also ensure the long term gains for all. Encouragement in employees: if the employees are encouraged through different methods like training, rewards or compensation, the sustainable HR capabilities can be built (Mazur, 2009). Long term employment: long term employment helps in providing security to the employees and avoids the absenteeism and disruption in employees which builds sustainable human resources and their capabilities. Continuous development and learning: This should be well planned which includes training programs and other evaluations which promotes the transfer of knowledge and builds sustainable HR capabilities (Breznik, 2014). Understanding HR and working on the competencies: The key feature of sustainable HR capabilities is how well the HR department of the organisation understand its human resources and work on their competencies. Assessment of strengths and Weaknesses: one of the important aspects of sustainable HR capability is that the strengths and weaknesses of the human resources should be assessed to work on their weaknesses and turning them into strengths (Khandekar Sharma, 2005). Future requirements of HR: the future requirements of HR need to be assessed in order to create value for future. Providing value: providing value to the human resources and adopting the powerful approach of being proactive for the employees builds up capabilities in HR sustainably (Shipton, Budhwar Crawshaw, 2012). For building sustainable HR capabilities the organisation needs to have integrated approach of handling the issues and providing solutions by keeping all the factors in mind. This will develop a fair and transparent culture in the organisation and will benefit in providing satisfaction to the employees. This will develop the employee commitment in the organisation for longer term benefitting the organisation and the future challenges of HR will be met (Tan Nasurdin, 2011). Conclusion It can be concluded that human capital is the key part of the organisation which is to be handled well in order to grow and develop the business in future. It is clear from the analysis of case that the hospital which does not have any separate HR department is losing its employees commitment, performance and growth. It is HR department which takes care of the most powerful asset of any organisation and improve their functional competency. The sustainable HR capabilities can be built by taking care of the needs of HR, valuing their contribution to the firm and continuous development and training. References Bloom, N and Van Reenen, J (2011). Human resource management and productivity,Handbook of labor economics,4, pp.1697-1767. Breznik, L (2014). Deploying human resource capability for sustainable performance: a dynamic capabilities framework. InHuman Capital without Borders: Knowledge and Learning for Quality of Life; Proceedings of the Management, Knowledge and Learning International Conference 2014(pp. 561-570). ToKnowPress. Guest, D E (2011). Human resource management and performance: still searching for some answers.Human resource management journal,21(1), 3-13. Khandekar, A Sharma, A (2005). Managing human resource capabilities for sustainable competitive advantage: An empirical analysis from Indian global organisations.Education+ Training,47(8/9), 628-639. Kramar, R (2014). Beyond strategic human resource management: is sustainable human resource management the next approach?.The International Journal of Human Resource Management,25(8), 1069-1089. Mazur, B (2009). Sustainable Human Resource Management in theory and practice.Economics and Management . Schroeder, H (2012). The importance of human resource management in strategic sustainability: An art and science perspective.Journal of Environmental Sustainability,2(2), 4. Sendogdu, A A, Kocabacak, A Guven, S (2013). The relationship between human resource management practices and organizational commitment: A field study.Procedia-Social and Behavioral Sciences,99, 818-827. Shipton, H, Budhwar, P S Crawshaw, J (2012). HRM, organizational capacity for change, and performance: A global perspective.Thunderbird International Business Review,54(6), 777-790. Stankeviciute, Z, Savaneviciene, A (2013). Sustainability as a concept for human resource management.Economics and Management,18(4), 837-846. Tan, C L Nasurdin, A M (2011). Human resource management practices and organizational innovation: assessing the mediating role of knowledge management effectiveness.Electronic Journal of Knowledge Management,9(2), 155-167. Tiwari, P Saxena, K (2012). Human resource management practices: A comprehensive review.Pakistan business review,9(2), 669-705.

Amway India Factsheet Essay Example

Amway India Factsheet Essay Amway promotes individual entrepreneurship through its innovative direct selling approach of world class consumer products. Amway India is the country’s leading direct selling FMCG-company which manufactures and sells world-class consumer products. Its business opportunity and all its products are  Ã‚  covered by  100% Money Back Guarantee. Amway sources all its products from within India, thereby providing stimulus to the local manufacturing industry. CORPORATE CREDENTIALS †¢ Amway India is a wholly owned subsidiary of US $ 7. billion Amway Corporation, Ada, Michigan, USA. Amway Corporation is one of the largest Direct Selling companies in the world. It has a presence in 80 countries territories. †¢ Established in 1995, Amway India commenced  its commercial operations in May 1998 and has emerged as the largest Direct Selling FMCG Company. The Company is headquartered at the National Capital Region of India New Delhi. †¢ Amway has invested in excess of U S $ 35 million (Rs. 151 crore) in India of this; US $ 6 million (Rs. 26 crore) is in the form of direct foreign investment. Amway India has 400 full time employees and has generated indirect employment for 1,650 persons at all the contract manufacturer locations. †¢ The Company has provided  income generating opportunity to over 4,50,000 active independent Amway Business Owners. †¢ Amway India provides free and unlimited training to all its distributors to help them grow their business. Amway India conducted over 29,000 training sessions during in the past 12-months with an attendance of over 1. 5 million Amway Business Owners and prospects. Amway India recorded a sales turnover of over Rs. 800 crore during January’07 –December’07. †¢ Amway India is a member of the Indian Direct Selling Association (IDSA). The IDSA is an industry regulatory body, with several reputed international and Indian Direct Selling companies as members. †¢ Amway India is also a member of the Confederation of Indian Industries (CII) and Federation of Indian Chambers of Commerce (FICCI). †¢ The World Blind Union presented an award and citation to Amway India in 2003, for its peerless work for the blind children. NATIONAL P RESENCE We will write a custom essay sample on Amway India Factsheet specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Amway India Factsheet specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Amway India Factsheet specifically for you FOR ONLY $16.38 $13.9/page Hire Writer In ten years of commercial operation, Amway India has established a nation-wide presence in over 125 offices and 55 city warehouses and four regional mother warehouses. The distribution and home delivery network set up with the support of independent logistics partners is spread across over 3,000 locations. MANUFACTURING Almost all Amway India products are manufactured in the country through 7 third party contract manufacturers. To bring the identified contract manufacturers’ production facilities and skills to international standard, Amway has invested in excess of US$ 4 million (approx. Rs. 17 crore). The transfer of this state-of-the-art, world-class technology, has been free of cost. PRODUCTS †¢ At present, Amway India offers over 105 products in four categories. They are Personal care category, Home Care category, Nutrition Wellness category and Cosmetics category. †¢ With the exception of Cosmetics range (Artistry*) and some products in Nutrition and Wellness category, all Amway India products and bottles are manufactured in India. †¢ The products match Amway’s global quality standards. They carry a tamper-proof seal and a ‘100% Money Back Guarantee. If not completely satisfied with the product, the consumer can return it for a refund. Amway products are environment friendly, and are not tested on animals. Amway encourages the return of its used product bottles for re-cycling and to prevent their misuse. CORPORATE RESPONSIBILITY AMWAY OPPORTUNITY FOUNDATION (AOF) †¢ Amway Opportunity Foundation (AOF) a registered non-profit organisation which looks after Amway India’s Corporate Social Responsibility (CSR). Amway distributors are also volunteers of AOF and they have enthusiastically participated in all AOF activities. Globally, Amway’s CSR campaign is known as the One By One Campaign for Children. PROMOTING FREE ENTERPRISE AND SELF-EMPLOYMENT †¢ Amway has conducted several seminars on ‘Entrepreneurial Development and Direct Selling’ in concert with the Confederation of Indian Industries (CII) and the All India Management Association (AIMA) on the benefits of organised direct selling. MEMBER IDSA (Indian Direct Selling Association) †¢ Amway India Enterprises Pvt Ltd  is a member of the India Direct Selling Association. The Indian Direct Selling Association is an association of companies engaged in the business of direct selling in India. Its members are of high national and international repute having set standards in delivering quality goods and in following ethical business practices.

Film and Indian Movies Essays

Film and Indian Movies Essays Film and Indian Movies Essay Film and Indian Movies Essay we do not have that much power and enthusiasm and also time to watch movies. youth as a grown ups,they should make themselves to grasp good things in a movie. Movies has a power to make the world and break the world. Indian actors and Indian movies are very much popular in Pakistani youth. Bollywood is one of the largest showbiz industries of the world. In Pakistan the Bollywood movies are very much famous and the real reason of Indian movies popularity is the gradually downward of our Pakistani showbiz industry. It is said that Urdu language became famous all over the world because of Indian Movies. Indian movies are promoting Indian culture and you would obviously know that all the Indian movies are based on Hindu mythology. Indian movies and Indian dramas are great source for the promotion of Indian culture. Experts believe that if you want to dominant on a nation you should capture their minds through propaganda and cultivate your own culture in their lives. Indian movies are doing the same thing with Pakistani nation. It is very famous incident of our daily life on marriages children mostly ask that husband didn’t wore â€Å"Mangal Sotar† so the marriage is still incomplete. Here we will discuss the overall impact of Indian movies on Pakistani youth as well as on the other people who watch Indian movies. First of all I would like to appreciate some Indian movies that nicely convey some public messages and highlighted some major issues. Munna Bhai in which Sanjay Datt conveyed a beautiful message that we can get our rights without any illegal methods. There are lots of ways rather than illegal ways to get your rights which he named â€Å"Gandi Geeri†. Similarly 3 Idiots is one of the beautiful movie in which they highlighted the flaws of educational system and also introduced new strategies for the betterment of education. â€Å"My Name is Khan† is also one of the best movies. But there is great number of Indian Movies that which are responsible for the promotion of crimes in the society. Most of the action movies are based on a same theme that someone do something bad with a person or family and in the response the hero of the movie started to take revenge. This common theme mostly conveys that in society you should be fight for your rights and mostly you should kill the people. The experts believe that the movies are very much influential on the daily life of people and most of the young boys and girls wanted to be like the hero of different Indian movies and they also wanted to cross the limitations

The Epistmology of Conspiracy Theories Essay Example | Topics and Well Written Essays - 1250 words

The Epistmology of Conspiracy Theories - Essay Example What exists about such events is only fantasizing of what might have been the cause. There are various such conspiracies in the world. The first one concerns the source of Human Immunodeficiency Virus that causes Acquired Immune Deficiency Syndrome. People speculate that it was a product of Soviet or American biological warfare and that it was released either with or without the knowledge of the scientist who made it. People were also not aware that such virus existed. The second conspiracy concerns extraterrestrials that frequently visited the earth with the intention of killing cows and human beings. The memories of these individuals and animals are then erased from our memories such that forget them forever (Keeley 6). The third conspiracy explains that there once had a good transport system in Los Angeles city which was made up of street cars. In 1930 to 1940, car manufactures, rubber makers and oil marketers formed a conspiracy group that distracted this transport system. The in tention of the group was to turn the city’s transport system into an automobile type. It, therefore, made large sales by selling such automobiles. The fourth conspiracy is that of â€Å"Trans World Airlines Flight 800.† The plane was accidentally sunk into the sea by a United State navy missile. The government then covered the facts behind this accident by refusing to release the right information to them media. These were just the mere speculations that went around in the public. The sixth most common conspiracy is the most surprising one. The United State is said to record all telephone calls made by people living in Transatlantic. The National Security Agency was the body behind this monitoring and recording (Keeley 8). Their primary intention is still yet to be known. The fifth conspiracy speculates that a small group of Jewish bankers controls the world economy. Other groups that were speculated were the Trilateral Commission and Freemasons. The sixth and the most talked about conspiracy involve the Oklahoma City bombing. It is speculated that a police officer guarding the main entrance of the building had in the previous days killed a person. His friends swore to revenge by throwing a bomb at him. They did so and killed the officer together with an extra 168 people. A clear analysis of the Oklahoma City bombing can reveal some characteristics of a conspiracy theory. After the bombing, news went around that the Bureau of Alcohol, Tobacco and Firearms had been forewarned before it took place, which they denied. A wise person should understand that such speculations or news in the media may be false or true. However, a conspiracy can be identified by a number of characteristics (Keeley 10). First, it offers the clarification of the event in question. The aim of this clarification is to confuse people’s minds and distract them far away from the truth. The second characteristic is that the people behind the bad event are revealed as weak and not capable of doing such an event. They are revealed to have played a helping role. They are seen as setting the main event that caused the disaster. Third, the group that does wrong or harmful event must be small in number. Other larger groups of people whom they are acting for, is largely hidden. Fourth, they cast doubts on an official story that had been earlier released. It, therefore, acts as a cover

International Business Plan for a Distance Education Company Term Paper

International Business Plan for a Distance Education Company - Term Paper Example Proven alternative research into alternative medicine is now available on numerous ailments including enhancement of longevity, safer hormone treatments, cancer treatment without radiation or chemotherapy, and normalization of diabetes induced high sugar levels (Goldstein 55). Most doctors in the developing world do not have training, time, or staff resources to handle alternative medicine in the developing countries. Most providers of health care have trouble keeping up with professional reading without having to learn more regarding alternative medicines. Patients in developing countries, which now are the fastest growing market for internet-enabled mobile phones, are increasingly turning to the internet for information on symptoms and diseases (Keegan 67). At the grass-root level, demand for alternative medicine driven by patients before the disease gains pace has gained momentum over the last few years. Leading futurist medicine advocates, including Dr. Eisenberg of Harvard Medic al School, point to various factors regarding the future of medicine in the developing world. The consumer now drives totally the demand for physical well-being and CAM is health care’s next wave. Additionally, CAM shows all the signs of social reform (Keegan 67). Resulting from this demand and new information supply, the internet-literate consumers of health care are now ahead of health care providers (Rakel et al 22). â€Å"Traditional† medicine is attempting to catch up to the consumer. SIL aims to become a vital and important provider of information to academic institutions, governments, and corporations. The World Wide Web and the internet are vital in the delivery of information seamlessly and instantly around the developing world to B2B clients, who... This paper approves that most doctors in the developing world do not have training, time, or staff resources to handle alternative medicine in the developing countries. Most providers of health care have trouble keeping up with professional reading without having to learn more regarding alternative medicines. Patients in developing countries, which now are the fastest growing market for internet-enabled mobile phones, are increasingly turning to the internet for information on symptoms and diseases. The World Wide Web and the internet are vital in the delivery of information seamlessly and instantly around the developing world to B2B clients, who are in need of providing this information that consumers seek so eagerly. Courseware in E-learning is a key system of delivery in the training of professionals in academic institutions, government, and corporations in the provision of accurate information on CAM. E-learning is ranked as the most promising and fast growing market segment in t he education industry. The primary target markets for SIL’s E-learning products and services are inclusive of four vertical markets. This essay makes a conclusion that this business plan is an E-learning and internet venture that is dependent on the financial prospects of developing countries and their parallel growth in the E-learning and internet world. In order for it to work financially, SIL has to increase schedule valuation in order to bring in added capital. The tables below define investor’s investing offerings.

Plant DCL Proteins

Plant DCL Proteins Abstract Dicer, a double-stranded RNA (dsRNA)-specific endoribonuclease, plays an essential role in triggering both transcriptional and post-transcriptional gene silencing in eukaryotes by cleaving dsRNAs or single-stranded RNAs bearing stem-loop structures such as microRNA precursor transcripts into 21- to 24-nt small RNAs. Unlike animals, plants have evolved to utilize at least four Dicer-like (DCL) proteins. Extensive genetic studies have revealed that each DCL protein participates in a specific gene silencing pathway, with some redundancy. However, a mechanistic understanding of how the specific action of each DCL protein is regulated in its respective pathway is still in its infancy due to the limited number of biochemical studies on plant DCL proteins. In this review, we summarize and discuss the biochemical properties of plant DCL proteins revealed by studies using highly purified recombinant proteins, crude extracts, and immunoprecipitates. With help from co-factor proteins and an ATP ase/DExH-box RNA-helicase domain, the microRNA-producing enzyme DCL1 recognizes bulges and terminal loop structures in its substrate transcripts to ensure accurate and efficient processing. DCL4 prefers long dsRNA substrates and requires the dsRNA-binding protein DRB4 for its activity. The short-dsRNA preference of DCL3 is well suited for short-RNA transcription and subsequent dsRNA formation by coupling between a plant-specific DNA-dependent RNA-polymerase IV and RNA-dependent RNA-polymerase 2 in the transcriptional gene silencing pathway. Inorganic phosphate also seems to play a role in differential regulation of DCL3 and DCL4 activities. Further development of biochemical approaches will be necessary for better understanding of how plant DCL proteins are fine-tuned in each small RNA biogenesis pathway under various physiological conditions. Introduction RNA silencing, also known as RNA interference (RNAi), is one of the fundamental molecular mechanisms conserved in most eukaryotes to regulate gene expression both transcriptionally and post-transcriptionally. In both situations, what triggers the RNA silencing pathway is a small RNA molecule, 21 to 24 nt in length, called small interfering RNA (siRNA) or microRNA (miRNA) depending on its origin and the downstream pathways involved. The class 3 endoribonuclease (RNase) III enzymes known as Dicer are responsible for producing siRNA from longer double-stranded RNAs (dsRNAs) and miRNA from single-stranded RNAs with internal stem-loop structures   by a dsRNA-specific endoribonuclease. Therefore, the activity and regulation of Dicer-family proteins in a cell are vital to many biological processes requiring flexible adjustments at the level of gene expression, such as development, organogenesis, the circadian rhythm, biotic and abiotic stress responses, and defense against viruses an d transposons. Biochemical characterization of Dicers in animals The Dicer family is a unique class of RNase III enzymes due to the presence of an ATPase/DExD/H-box helicase domain at the N-terminus, a Piwi/Argonaute/Zwille (PAZ) domain in the middle and dual RNase III domains followed by one or two dsRNA-binding domains in the C-terminal half (exception: Giardia intestinalis) (Figure 1) (Bernstein et al. 2001). In general, the helicase domain serves as a protein-protein interaction surface recruiting co-factor regulatory proteins (Lee et al. 2006; Ma et al. 2008; Ye et al. 2007). It also utilizes ATP hydrolysis to achieve processive cleavage of the long dsRNA substrate (Cenik et al. 2011; Welker et al. 2010). The PAZ domain contains a conserved pocket for recognizing the terminus of the dsRNA substrate, and the distance between PAZ and the RNase III catalytic center determines the product sizes (MacRae et al. 2007; MacRae et al. 2006). Each of the two RNase III domains cuts one of the dsRNA strands, leaving a characteristic 2-nt overhang at 3-end of the product (Elbashir et al. 2001; Takeshita et al. 2007; Zhang et al. 2004). The C-terminal dsRNA-binding domains (dsRBDs) serve as a protein-protein interaction interface and nuclear localization signals, in addition to having dsRNA-binding function (Doyle et al. 2013; Hiraguri et al. 2005; Wostenberg et al. 2012). The specific functionality of each domain differs depending on the Dicer protein. Since the first demonstration of in vitro small RNA-producing activity of Dicer in the fruit fly Drosophila melanogaster (Bernstein et al. 2001), its biochemical properties and regulatory machinery have been extensively studied in humans, D. melanogaster and Caenorhabditis elegans. In humans, there is only one Dicer-family protein (hDicer), which cleaves short-hairpin pre-miRNAs produced by Drosha and dsRNA substrates into 20- to 22-nt small RNAs in an ATP-independent manner (Myers et al. 2003; Provost et al. 2002; Zhang et al. 2002). The cleavage activity requires a divalent metal cation such as Mg2+, Co2+ or Mn2+, and recognizes mainly the 5-end of the substrate to dictate the product length (Park et al. 2011). This 5-counting rule is reliant on the conserved 3-pocket motif within the PAZ domain and the 5-pocket motif, which is less conserved in Dicers of other eukaryotes. The binding of Dicer to a dsRNA substrate and its cleavage are uncoupled, because Dicer can bind to dsRNA with out Mg2+ or under low temperature (Provost et al. 2002; Zhang et al. 2002). The helicase domain of hDicer has an autoinhibitory function (Ma et al. 2008). In line with this, the activity of recombinant full-length hDicer protein can be improved under limited proteolytic conditions (Zhang et al. 2002). hDicer is responsible for both siRNA and miRNA production, and co-factor dsRNA-binding proteins TRBP and PACT dictate hDicer function in the two distinct small RNA production pathways (Chendrimada et al. 2005; Haase et al. 2005; Kok et al. 2007; Lee et al. 2013; Lee et al. 2006). In particular, the hDicer complex containing PACT disfavors siRNA precursor dsRNA and shows different cleavage patterns on the same pre-miRNA substrate than the hDicer-TRBP complex (Lee et al. 2013). The interaction with TRBP occurs through the hDicer helicase domain, and stimulates the hDicers catalytic activity. (Ma et al. 2008). Similarly, it has been reported that the C. elegans Dcr-1 interacts with a dsRNA-binding protein RDE-4 which enhances the Dicer activity toward long dsRNA substrates in siRNA production, while RDE-4 is apparently dispensable in miRNA production pathway (Parker et al. 2006; Parker et al. 2008; Tabara et al. 2002). D. melanogaster has two Dicer proteins, Dcr-1 and Dcr-2, which produce miRNA and siRNA, respectively (Lee et al. 2004; Miyoshi et al. 2010). Dcr-1 alone can process dsRNA into siRNA in vitro, but its interaction with the dsRNA-binding protein Loquacious isoform PB (Loqs-PB) confers pre-miRNA substrate specificity to the Dcr-1-Loqs complex by suppressing cleavage of long perfect dsRNAs and enhancing pre-miRNA processing activity (Saito et al. 2005; Zhou et al. 2009). Dcr-2 interacts with Loqs isoform PD and another dsRNA-binding protein, R2D2, in the siRNA production pathway (Liu et al. 2003; Liu et al. 2006; Miyoshi et al. 2010; Zhou et al. 2009). Dcr-2 alone is also capable of cleaving a pre-miRNA precursor in an ATP-independent manner, but R2D2 significantly suppresses Dcr-2 activity toward pre-miRNA, whereas Loqs-PD enhances the cleavage activity of Dcr-2 toward long perfect dsRNA precursors by boosting its affinity to the substrate (Cenik et al. 2011; Miyoshi et al. 2010). The pr ocessive processing of long dsRNA substrates by Dcr-2 depends on ATP hydrolysis by its ATPase/helicase domain, implying that one of the functions of the helicase domain is to allow Dcr-2 to produce multiple siRNAs from a single long dsRNA molecule before it dissociates from the substrate (Cenik et al. 2011). Such differential regulation of Dicer activity through specific interaction with co-factor dsRNA-binding proteins in distinct pathways is commonly found in most of the systems studied, including plants. DCL proteins in plants Plant genomes contain at least four distinct classes of DCL family proteins (DCL1-4). Like their animal counterparts, each class of DCL has evolved to participate in its primary pathway (Figure 2), but the three siRNA-producing DCLs (DCL2-4) function redundantly as well, because defects in one class of DCL can be compensated for by other classes in some cases (Gasciolli et al. 2005; Mukherjee et al. 2013; Xie et al. 2004). Because DCL1 is the only Dicer protein that produces most 21-nt miRNAs (Kurihara and Watanabe 2004; Reinhart et al. 2002), knockout mutants of DCL1 are embryonic lethal (Schauer et al. 2002). DCL4 is the major producer of 21-nt antiviral siRNA and endogenous siRNAs such as trans-acting siRNA and phased siRNAs (phasiRNA) (Bouche et al. 2006; Gasciolli et al. 2005; Mukherjee et al. 2013; Qu et al. 2008; Xie et al. 2005; Yoshikawa et al. 2005). DCL2 can compensate for the loss of DCL4 (Bouche et al. 2006; Gasciolli et al. 2005; Parent et al. 2015), although its major function remains unclear. DCL3 mainly produces 24-nt repeat-associated siRNAs derived from transposons and DNA repetitive elements, and participates in transcriptional gene silencing (TGS) through RNA-dependent DNA methylation, suppressing proliferation of these elements (Henderson et al. 2006; Pontes et al. 2006; Xie et al. 2004). In addition to the four classes of DCLs, monocots have another distinct class of Dicer, DCL5 (also known as DCL3b) (Margis et al. 2006). DCL5 is specifically expressed in developing panicles and is responsible for 24-nt reproductive phasiRNAs, although the biological significance of a reproductive-organ-specific 24-nt phasiRNA pathway mediated by this specific Dicer remains to be elucidated (Borges and Martienssen 2015; Fei et al. 2013; Kapoor et al. 2008; Song et al. 2012). This pathway might be analogous to the Dicer-independent PIWI-interacting RNA (piRNA) pathway in vertebrates, which suppresses transposons and other genes specifically in germlines (H irakata and Siomi 2016). Both forward and reverse genetics and physiological studies have successfully dissected the major RNA silencing pathways and allowed identification of the function of DCL genes in each pathway in plants. However, investigations on the molecular and enzymatic characteristics underlying the functional diversification and specificity of the DCL proteins are still in their infancy. Detection of DCL activity in crude extracts of various plants Biochemical characterization of plant Dicer activity was first demonstrated in wheat germ extract (monocot) and cauliflower extract (dicot), which contain multiple DCL activities producing ~21 nt and ~24 nt small RNAs with 2-nt 3-overhangs in the double-stranded form (Tang et al. 2003). These activities are weaker in the absence of ATP, consistent with characteristics of Dicer family proteins from Drosophila and C. elegans. Long dsRNA competitors effectively suppress both activities in wheat germ extract. The 24-nt small RNA producing activity was inhibited by 25-nt synthetic siRNA duplexes, whereas 21-nt small RNA production was unaffected by 21-nt synthetic siRNA duplex competitors, suggesting that two different enzymes with active sites that have distinct size-dependent binding properties are in the wheat germ extract (Tang et al. 2003). A recent study on wheat germ extract characterized these activities in further detail, revealing (1) that the 21-nt activity could be found in a much larger (~950 kDa) complex than the 24-nt activity, which had maximum activity in an approximately 450 kDa complex; and (2) the biochemical properties associated with the activities, such as divalent cation and NTP requirements, optimum NaCl concentration, temperature, and pH, and substrate length dependence (Shivaprasad et al. 2015). The identities of the DCL enzymes responsible for these activities in the wheat germ extract remain to be identified. A better understanding of the biochemical characteristics of individual plant Dicer proteins has come from the model plant Arabidopsis thaliana, which has four DCL proteins: DCL1, DCL2, DCL3 and DCL4 (summarized in Table 1). The first in vitro DCL activity in A. thaliana was demonstrated using a suspension cell lysate, a crude extract of inflorescence tissue, and an immunoaffinity-purified protein complex (Qi et al. 2005). Similar to the previous study using wheat germ extract or cauliflower, extracts from both Arabidopsis cultured cells and inflorescence tissue contained DCL dsRNA-cleaving activity producing 21- and 24-nt small RNAs from 400-bp dsRNA (Qi et al. 2005). The 21-nt producing activity and 24-nt producing activity were found in >660 kDa and ~400 kDa fractions, respectively, suggesting that these Dicers reside in protein complexes composed of multiple co-factors (Qi et al. 2005). In agreement with previous genetic studies showing CARPEL FACTORY/DCL1 is responsible for 21-nt miRNA production in vivo (Kurihara and Watanabe 2004; Reinhart et al. 2002), the 21-nt small RNA producing activity was DCL1 immunoaffinity-purified from inflorescence-derived crude extract by an anti-DCL1 antibody (Qi et al. 2005). The 24-nt activity was associated with anti-DCL3 antibody immunoprecipitate, and the activity was abolished when purified from a dcl3-1 mutant, showing that DCL3 is responsible for the 24-nt activity in Arabidopsis inflorescence extract. The immunoaffinity-purified DCL1 activity required ATP, whereas the activity of the DCL3 immunoprecipitate was ATP-independent (Qi et al. 2005). Interestingly, the dcl1-7 mutation did not abolish the 21-nt small RNA producing activity in the extract or immunoprecipitates, implying that the substitution (P415S) in its N-terminal helicase domain did not alter the enzymes catalytic activity itself (Qi et al. 2005); this study also found that the activity of DCL4 responsible for formation of 21-nt siRNA was present in the inflorescence extract. The presence of DCL4 activity in an Arabidopsis crude extract was demonstrated in later studies using 2-week-old seedlings as the starting material (Fukudome et al. 2011; Nagano et al. 2014), and will be discussed later in this review. In-depth biochemical characterization of DCL1, a microRNA-producing enzyme in plants  DCL1 activity requires DRB1/HYL1 and SERRATE for accurate processing of the miRNA precursor Both in wheat germ and Arabidopsis extracts, DCL activities are associated with size fractions larger than DCL monomeric form, implying that these DCLs form functional protein complexes composed of multiple co-factors in vivo. As summarized in an earlier section, such interactions between a Dicer and a co-factor protein are commonly found in mammals, nematodes and insects. One of the most characterized classes of co-factor proteins is a dsRNA-binding protein (dsRBP) harboring multiple dsRNA-binding domains or motifs. The A. thaliana genome encodes five dsRNA-binding (DRB) family proteins: DRB1/HYL1, DRB2 DRB3, DRB4, and DRB5. Multiple genetic and biochemical studies have demonstrated two specific interactions between DCLs and DRBs in A. thaliana: DCL1-DRB1/HYL1 and DCL4-DRB4 (Han et al. 2004; Hiraguri et al. 2005; Kurihara et al. 2006; Nakazawa et al. 2007). Arabidopsis DCL1, DRB1/HYL1, and another co-factor, SERRATE (SE), constitute an essential microRNA production pathway in vivo (Han et al. 2004; Lobbes et al. 2006). Unlike animals, which utilize two distinct RNase III enzymes, Drosha and Dicer, for the first and second cleavage of microRNA precursors, plants do not employ Drosha. Therefore, the DCL1-complex is responsible for the processing of both primary and precursor miRNA substrates. The detailed molecular machinery of the dual miRNA processing mediated by DCL1 and the co-factor proteins have been extensively studied biochemically using highly purified recombinant proteins produced in heterologous systems (summarized in Figure 3). One of the systems utilizes baculovirus-mediated recombinant protein production in Sf21 insect cells, followed by two-step affinity purification (Dong et al. 2008). The highly purified recombinant DCL1 protein alone could process a 94-bp dsRNA substrate with a 2-nt 3-overhang into 21-nt small RNA in an ATP/Mg2+ dependent manner. The optimum NaCl concentration for the activity was 25-50 mM, and a NaCl concentration higher than 100 mM severely impaired the activity (Dong et al. 2008). While the recombinant DCL1 protein alone could produce 21-nt small RNA from both primary and precursor miRNA (pri-/pre-miR167b) substrates in vitro, DRB1/HYL1 and SE recombinant proteins co-incubated in the same reaction mixtures significantly increased both yield and accuracy of the processing (Dong et al. 2008). Without these co-factors, more than 80% of 21-nt small RNA products from the DCL1-alone reaction were due to incorrect processing from the end of the primary miRNA substrate, whereas the processing mediated by the DCL1-DRB1/HYL1-SE complex produced accurate 21-nt products with a sequence identical to miR167b/miR167b*, amounting for up to 81% of the products (Dong et al. 2008). This demonstrated that accurate processing of miRNA precursors by DCL1 requires the co-factors DRB1/HYL1 and SE. Cons istent with a previous study, the interaction between DCL1-DRB1/HYL1 through the second dsRNA-binding motif of DCL1 is important for the precise processing of pri-miRNA in A. thaliana (Dong et al. 2008; Kurihara et al. 2006). Also, using highly purified recombinant proteins and surface plasmon resonance analysis, it has been suggested that DCL1 changes its structural conformation when it binds RNA and exposes more binding sites for SE (Iwata et al. 2013). Binding to substrate dsRNA or miRNA precursors might be an important regulatory step for DCL1 dicing activity, as its dsRNA-binding domains exhibit the strongest binding to dsRNA among the four Arabidopsis DCLs (Hiraguri et al. 2005). ATPase/DExH-box RNA-helicase domain of DCL1 suppresses its dicing activity, confers ATP dependence, and influences processing accuracy In addition to its RNase III and dsRNA-binding domains, the helicase domain of DCL1 plays a significant role in regulating its dicing activity. Two independent forward genetic studies have identified two dcl1 mutant alleles, dcl1-13 (E395K) and dcl1-20 (R363K), as hyl1 suppressors, and the amino acid substitutions of both alleles occur within the ATPase/DExH-box RNA-helicase domain. These dcl1 mutations partially rescue the accumulation of some miRNAs in a hyl1-2 mutant (Liu et al. 2012; Tagami et al. 2009), and dcl1-13 was at least partially able to restore the phenotypic defects of hyl1-2 such as a reduced number of rosette leaves and a   leaf shape (Tagami et al. 2009). Highly purified recombinant DCL1-20 protein exhibited enhanced catalytic activity (Kcat/Km) toward pri-miRNA156a compared to wild-type DCL1 (Liu et al. 2012). Similarly, the helicase domain-deleted DCL1 recombinant protein (DCL1à ¢Ã‹â€ Ã¢â‚¬  Helicase) showed higher processing activity in vitro and was no l onger dependent on ATP for its activity toward pri-miRNA156a (Liu et al. 2012), suggesting that the helicase domain of DCL1 might have an autoinhibitory function like that of human Dicer (Ma et al. 2008; Provost et al. 2002). The in vivo miRNA processing imprecision in hyl1-2, however, was not restored by a dcl1-20 mutation, implying that the partial recovery of the hyl1-2 mutant, including miRNA accumulation, was due to the enhanced catalytic activity resulting from the substitution in the helicase domain (Liu et al. 2012). Interestingly, the effect and magnitude of DRB1/HYL1 and DCL1 helicase domain seem to vary among miRNA precursors. For example, the in vivo processing accuracy of miR156a is much more severely affected by hyl1-2 mutation than miR166b is (Liu et al. 2012). pri-miR156a is processed from the loop-proximal site to the loop-distal base in vitro (Liu et al. 2012), which is considered unusual for plant miRNAs (Addo-Quaye et al. 2009; Mateos et al. 2010). Accurate processing of pri-miRNA166b by native DCL1 is largely dependent on the presence of ATP, and processing by DCL1à ¢Ã‹â€ Ã¢â‚¬  Helicase is less accurate than that of native DCL1 (Liu et al. 2012). In contrast to miR156a, the processi ng precision of which is markedly affected by hyl1-2, that of miR166b was much more impaired by dcl1-20 mutation than hyl1-2 (Liu et al. 2012). Also, the effect of the other helicase mutant allele, dcl1-13, on miRNA production was shown to depend on the presence or absence of DRB1/HYL1 in vivo (Tagami et al. 2009). These observations indicate that efficient processing of different miRNA precursors by DCL1 have different reliance upon DRB1/HYL1 and DCL1helicase domain that potentially depends on structural determinants of the miRNA precursors. Structural determinants for efficient and accurate processing of miRNA precursors by DCL1 Primary transcripts of miRNA (pri-miRNA) have a characteristic secondary structure: a loop-distal stem (lower stem), a miRNA duplex, a loop-proximal stem (upper stem) and a terminal loop (Figure 3). Typical miRNA maturation from these precursors requires at least two cleavages occurring at the lower and upper stems. In animals, the single-stranded base region of the loop-distal stem is recognized by the dsRNA-binding protein DGCR8, which guides the processing center of Drosha to the correct position, which is 11 nt from the base of the stem (Han et al. 2006). However, this distance-from-base rule is not sufficient for plants because the length of the loop-distal stem of plant pri-miRNAs is highly variable (Song et al. 2010). Several structural features of pri-miRNAs that influence the activity, binding position and directionality of the processing by DCL1 have been elucidated genetically and biochemically (Figure 3a, b). One structural determinant lies within the loop-distal stem of pri-miRNA. For the first cleavage at the loop-distal stem, bulges and unpaired regions play a major role in the efficiency of miRNA processing. Mutant pri-miRNAs with closed bulges were processed at the correct position, but resulted in the accumulation of unprocessed pre-miRNAs in vivo, indicating that the rate of subsequent processing at the loop-proximal stem was impaired (Song et al. 2010). In pri-miR171a, which has a long loop-distal stem, the first cleavage position was determined by the distance from a relatively unstructured region instead of the base of the stem; the conserved distance from an unstructured region of the lower stem important for miRNA processing was found to be approximately 15 nt (Figure 3a) (Mateos et al. 2010; Song et al. 2010; Werner et al. 2010). The 15-nt rule was essentially reproduced in an in vitro miRNA processing system using highly purified DCL1-DRB1/HYL1-SE recombinant proteins and an artificial pri-miRNA substrate bearing another unstructured region in the elongated lower stem. In addition to the canonical processing, another type of processing occurred at 15 nt from the artificially introduced unstructured region, validating the functionality of the 15-nt rule (Song et al. 2010). The importance of bulges and unpaired regions in the lower stem for processing by DCL1 might explain why some miRNAs with a near-perfect matched stem seem to be DCL4-dependent, rather than DCL1-dependent (Rajagopalan et al. 2006; Song et al. 2010). On the loop-proximal and terminal loop side, a branched terminal loop (BTL) or a large terminal loop was found to be an essential structural factor that may alter directionality of processing by DCL1 and the resultant miRNA-accumulation (Figure 3b). BTL induces abortive processing of pri-miR166c both in vivo and in vitro (Zhu et al. 2013), meaning the first cleavage of the pri-miRNA occurs in the loop-proximal stem as opposed to the normal productive processing beginning in the loop-distal stem. The molecular basis of this bidirectional processing by DCL1 was further investigated using an in vitro system that reconstitutes the DCL1-processing machinery. For this purpose, DCL1, DRB1/HYL1 and SE harboring Agrobacterium tumefaciens were co-infiltrated to Nicotiana benthamiana leaves, and the transiently expressed DCL1-DRB1/HYL1-SE complex was immunoaffinity-purified two days after infiltration (Zhu et al. 2013). The reconstituted DCL1 complex cleaves the substrate pri-miRNA 16-17 nt fro m the unpaired region of the lower stem, supporting previous studies (Mateos et al. 2010; Song et al. 2010; Werner et al. 2010). By disrupting one of the two RNase III domains of DCL1 alternately and using 5- or 3-end labeled pri-miR166c substrates, the bidirectional nature of both productive and abortive processing was demonstrated (Zhu et al. 2013; Figure 3b). The helicase domain of DCL1 fine-tunes the position of both productive and abortive processing by DCL1 in an ATP-dependent manner (Zhu et al. 2013). DCL1à ¢Ã‹â€ Ã¢â‚¬  Helicase complex could not abortively process a substrate with BTL. Also, wild-type DCL1 required ATP for abortive processing, but not productive processing, indicating that the ATPase-driven helicase activity is necessary in abortive processing to unwind the structured BTL (Zhu et al. 2013; Figure 3b). In productive processing, the effect of helicase deletion and ATP depletion depend on the distance between the processing site and the bulge in the lower stem. Many potential byproducts of the abortive processing of pri-miRNA precursors with BTL can be found in publically available high-throughput small RNA sequencing data from both Arabidopsis and rice, implying that both substrate structure and the functionality of the ATPase/helicase domain of DCL1 are conserved mechanisms to regulate miRNA biogenesis in higher plan ts (Zhu et al. 2013). Dissecting distinct characteristics of DCL3 and DCL4 activities DCL4 activity requires the dsRNA-binding protein DRB4 In A. thaliana, DCL2, DCL3 and DCL4 are responsible for producing various siRNAs 21-24 nt in length. The dsRNA-cleaving activities of DCL3 and DCL4 can be detected in crude extracts prepared from 2-week-old seedlings (Fukudome et al. 2011). Extracts from wild-type seedlings cleave 500-bp dsRNA substrates into 21-nt and 24-nt small RNAs. In this system, the 21-nt and 24-nt small RNA-producing activities can be attributed to DCL4 and DCL3 respectively, because the dsRNA-cleaving activity of the corresponding size was abolished in each of the single mutants (Fukudome et al. 2011). Also, a mutation in the dsRNA-binding protein DRB4, which interacts with DCL4 (Hiraguri et al. 2005; Nakazawa et al. 2007), abolished DCL4 activity in seedling extracts. The DCL4 activity could be further purified by immunoprecipitation with anti-DCL4 or anti-DRB4 antibodies. The immunoaffinity-purified DCL4 requires Mg2+ and ATP for its activity, and is inhibited by >200 mM NaCl. This property is similar to t hat of recombinant DCL1 protein (Dong et al. 2008). The DCL4 complex immunoprecipitated from the drb4-1 mutant did not show dsRNA-cleaving activity, but the addition of recombinant DRB4 protein to the complex restored the 21-nt producing activity in vitro, showing that DRB4 functions as an essential co-factor for the dsRNA-cleaving activity of DCL4 (Figure 4b). In this system, mutant DRB4 proteins harboring substitutions in the conserved amino acid residues that form a hydrogen bond with the phosphodiester backbone of dsRNA at the dsRNA-binding site (H32A in the first dsRBD and K133A in the second dsRBD of DRB4) lost their ability to interact with dsRNA and DCL4, and did not restore DCL4 activity. The second substitution (K133A) alone impaired its interaction with the C-terminal half of DCL4 containing two RNase III domains and two dsRBDs in a GST pull-down assay using recombinant proteins, but was not sufficient to block restoration of DCL4 activity when added to DCL4 immunopurified from a drb4-1 mutant extract. There might be an add itional interaction surface between DCL4 and DRB4 involving dsRBD1 of DRB4 and the N-terminal half of DCL4, which contains an ATPase/DExH-box RNA-helicase domain and an RNA-binding domain (formerly known as domain of unknown function DUF283; Figure 1), as their specific interaction was reported in vitro (Qin et al. 2010). Short dsRNA preference of DCL3 activity orchestrates 24-nt siRNA biogenesis in TGS pathway Crude extracts from 2-week-old seedlings have also been used to characterize substrate specificity of DCL3 and DCL4. Consistent with the long dsRNA preference of Drosophila Dcr1 (Bernstein et al. 2001), DCL4 preferentially cleaves longer dsRNA substrates, and is less efficient in producing 21-nt siRNAs when the substrate is shorter than 50 nt (Nagano et al. 2014). On the other hand, DCL3 activity, producing 24-nt siRNAs, favors shorter substrates such as 30 nt and 37 nt dsRNA with a 1-nt or 2-nt 3-overhang (Nagano et al. 2014). It also favors substrate dsRNA with 5-adenosine or uridine. The 24-nt small RNA produced by DCL3 has a 2-nt 3-overhang, and the cleavage follows the 5-counting rule proposed for human Dicer (Park et al. 2011). DCL3 is not reliant on ATP hydrolysis for activity, as it can still process the short dsRNA substrate in the presence of a non-hydrolyzable ATP analog, adenosine 5-O-(3-thio)triphosphate (Nagano et al. 2014). Unlike DCL4, which targets long dsRNAs such a s RDR6-dependent TAS dsRNAs or exogenous viral dsRNAs in vivo (Bouche et al. 2006; Dunoyer et al. 2005; Qu et al. 2008; Yoshikawa et al. 2005), DCL3 may not need to perform a processive cleavage, which requires ATP hydrolysis, because the length of its targets allows only a single cut (Figure 4a). The DCL3 preference for short dsRNA substrate is consistent with the one precursor, one siRNA model for RNA polymerase IV (Pol IV)-dependent 24-nt siRNA biogenesis (Blevins et al. 2015; Zhai et al. 2015). In this model, a remarkably short (30- to 40-nt) transcript with 5-adenosine is produced by Pol IV and is simultaneously converted into double-stranded form by an RNA-dependent RNA polymerase, RDR2. The short dsRNA substrate is processed into 24-nt siRNA preferentially by DCL3 due to its length specificity, facilitating the subsequent RNA-directed DNA methylation process (Blevins et al. 2015; Zhai et al. 2015). The transcription of short RNAs by Pol IV, and the length and 5-adenosine substrate preference of DCL3 might be essential mechanisms to prevent other DCLs from processing specific dsRNA substrate needed for the TGS pathway. Such coupling of RDR-Dicer-RNAi is also known in fission yeast, where a Dicer physically interacts with an RNA-dependent RNA polymerase to form coupled ma chinery that drives siRNA-mediated TGS (Colmenares et al. 2007). In addition, DCL3 can participate in 24-nt siRNA production from longer transcripts with aid from another RNase III enzyme, RNase III-like 2 (RTL2). As a class II RNase III enzyme, RTL2 possesses one RNase III domain and two dsRBDs, and is involved in rRNA maturation [in vivo is implied]in A. thaliana (Comella et al. 2008). Recombinant RTL2 protein can cleave long dsRNA substrates into 25 bp or longer dsRNA in vitro (Kiyota et al. 2011). Recently, it has been shown that RTL2 processes a subset of Pol IV-dependent dsRNA into shorter intermediates, which are preferable for DCL3 activity in vivo (Elvira-Matelot et al. 2016). Although no direct interaction has been reported, RTL2 and DCL3 can be considered other examples of coordinated action of a dsRBD-containing protein and a Dicer in plants. DCL3 is also reported to physically interact with the dsRNA-binding protein DRB3 in the antiviral RNA-directed DNA methylation pathway (Raja et al. 2014). The function of DRB3 in DCL3 activity rem ains elusive. Inorganic phosphate, NaCl and KCl differentially regulate DCL3 and DCL4 activities In the same assay system using crude extracts, inorganic phosphate at a physiological concentration promotes DCL3 activity but suppresses DCL4 activity toward 50-nt dsRNA substrates (Nagano et al. 2014). The differential effe

Automobile and Level Material Appendix Essay

Choose one of the Facts for Consideration sections from Ch. 3 of the text and list the page number for the section you chose. Then, complete the following table. List five threats appropriate to the environment from the section you chose. Rate the risk for each threat from 0 (low) to 10 (high). Then, list five appropriate countermeasures. Once you complete the table, write a brief explanation of the countermeasures for the two threats with the highest risk total, stating how the countermeasure reduces the risk associated with that threat. This assessment is based on the Facts for Consideration on page _93___ THREAT| RISK| COUNTERMEASURE| | Probability| Criticality| Total| | Example:Physical assault| 9| 4| 13| Highly visible officer presence| Inmate Escape| 10| 5| 15| Extra guards to help prevent escape attempt| Inmate Riots| 8| 4| 12| Officers carry visible weapons to show that if inmates get out of hand measures will be taken |. Transport vehicle driver exhausted | 9| 4| 13| Extra driver to transport vehicle in addition to other guards that aren’t driving| Inmates take over the van| 10| 4| 14| Have the inmates checked before they get on the van and then handcuffed so they are not able to move around or get close to the driver| Have more than one officer on the van | 8| 3| 11| One officer can drive and the other can keep an eye on the inmates|. The inmate escape is very important. If there are extra guards then it will be easy to prevent this from happening, especially since some trips can take up to 48 hours to complete. With all the stopping (two to eight stops on any given trip) and going it would be great to have the countermeasure. When it comes to inmates taking over the van, I feel as though that is something that can happen even if there are two officers on the van. Inmates are strong and they always have something up their sleeves. So if we were to take the countermeasure and actually have them checked before they get on the van and then handcuffed it makes the ride safer and less threatening.

Postmodern and Hyper Reality

Modern Culture and Hybridization PAPER #1 3/18/2013 HUM310-09 Daniel Echeverria The world has drastically changed since the beginning of online dating and other social network sites. With the growing number of people getting connected in the network, the way older generations used to communicate with each other is slowly diminishing. Today’s social networking sites have altered the way we communicate with each other, changed the ways that we evaluate one another, and the way we express ourselves.Due to the vast amount of information that can be found on the internet, a person can know about another’s past, current social circle, and interests in a matter of minutes. This has completely changed the way our generation was taught to interact with each other. For so many people now-a-days it has also become part of their social identity. Characteristics and pictures may be exaggerated slightly or completely fabricated, with the intention of enhancing one’s identity f or outside observers.As more innovations in the cyber social network are developed, today’s post-modern culture society is heading toward an hyper reality. According to â€Å"Statistic Brain† a US statistics website, there are currently 50 million people single in the United States. 40 Million People have tried online dating.? The way we meet and interact with one another isn’t the same as was 30 years ago. Our generation was taught different on the subject of dating and â€Å"Getting to know† someone. What used to be the only way of building a relationship or dating is now changed with the amount of information that is on the internet.We have seen that in films, a man meets a woman, they have face-to-face interactions, they talk about themselves and who they are and what they want to become. 1. Statistic Brain â€Å"Online Dating Statistics†, online http://www. statisticbrain. com/online-dating-statistics (6/20/2012) Relationships started with get ting to know someone unknown, communicating with that someone, and building a relationship out of the experiences with them. Due to the growth of online dating and other network sites, a person can be reviewed before they can present themselves.Websites like Facebook & MySpace give the user a profile where they can give a description of who they are without directly telling anyone. Someone with a user profile in these websites can be looked up in within seconds just by typing in the other person’s name in a regular web search engine. The excitement of exploring the person’s personality quickly vanishes with a stroke of a button. What was once local pub, or local cafe, or night club as the only ideal destination for social networking has now become online dating websites and online social networks.Society has begun to adapt these dating methods by using the tools and information in the internet to evaluate one another prior to the actual dating. The traditional dating e xperience of discovery, surprise, and experience is slowly fading away. In Glenn Ward’s Teach Yourself series, Glenn talks about postmodernism and the changes we need to adapt to, â€Å"†¦ society, culture and lifestyle are today significantly different from what they were 100, 50 or even 30 years ago †¦. Old styles of analysis are no longer useful, and that new approaches and new vocabularies need to be created in order to understand the present. ? Due to the rapidly increasing information technology, and social network users, the 2. Ward, Glenn â€Å"Teach Yourself† (Teach Yourself, 1998) modern way to share and research anyone’s personal information is through the web. It can be can be used in many different ways, negative and positive. Law enforcement has used these social websites to investigate suspects and quickly have a better idea of who the person is. A users profile can quickly give brief information on where the user lives, interests, and social group.But it can also be used to leak viral information in seconds. A recent example was of a LAPD manhunt in February 2013. In early February 2013, a suspected criminal Christopher Dorner posted a manifesto exposing the violence of the LAPD in his Facebook profile. This information was instantly available to everyone to read and share before there was any attempt to destroy the manifesto. ? Another group that has adopted the social network sites as tools for evaluating individuals is employers. Now-a-days an employer can view an applicant’s profile to screen the individual.Or an employee can be affected by what he/she decides to put on their profile page. According to Oregonbuisness. com, a survey was conducted in Chicago in 2009, â€Å"Forty-five percent of employers reported in a recent CareerBuilder survey that they use social networking sites to research job candidates, a big jump from 22 percent last year. Another 11 percent plan to start using social networking sites for screening. More than 2,600 hiring managers participated in the survey, which was completed in June 2009. †? 3. Wikipedia, â€Å"Christopher Dorner† online http://en. wikipedia. rg/wiki/Christopher_Dorner (2/13/2013) 4. Oregon Business Report, â€Å"45% Employers use Facebook-Twitter to screen job candidates† online http://oregonbusinessreport. com/2009/08/45-employers-use-facebook-twitter-to-screen-job-candidates (8/24/2009) In other cases employees are fired for pictures they have posted and blogs they have written on their user profiles while on their personal time. It can be amazing how society has embraced and trusted information technology now when merely a few decades ago the public was nervous of having the same technology invade their privacy.Kimberly N Rosenfeld mentions this in her article Terminator to Avatar: A Postmodern Shift, â€Å"Cinematic representations of a society in shambles brought about by humankind’s losing control to i ts machines were popular with audiences in a period when the sociopolitical context fostered anxiety and technological mistrust. †? Although social networks provide the tools to the users so that they can express themselves, a user could use the profile to portray themselves as someone they are not.Not too long ago, punk rockers in the UK showed the world their rebellion through appearance and politically themed lyrics. During the 1980’s Iranian War, it was obligatory for young women to wear a veil at school. In Marjane Satrapi’s graphic novel â€Å"Persepolis† a young Iranian school girl identifies herself as a rebel by the way she dresses and the way she communicates her views. She dresses in public with a denim jacket, with Michael Jackson Pins and Nike shoes.Her image is that of a modern punk which was not tolerated from women at the time.? As mentioned earlier in this essay, a user’s personality can be now easily identified by viewing the userà ¢â‚¬â„¢s profile. Pictures, blogs, personal statements, comments on others pages, can easily describe 5. Rosenfeld N, Kimberly (Terminator to Avatar: A Post Modern Shift, 2010) 6. Satrapi, Marjane â€Å"Persopolis† (Random House Inc, 1969) who the user is. Society has adopted this form of self-expression and has become comfortable on showing their identity this way.The user has become so addicted to the world of online social networking that physical communication and real social networking is almost not preferred. Almost similar to a gamer feeling confident playing an online sport better than the actual sport itself. Rosenfeld also describes this while comparing the movie â€Å"Avatar† to modern hyper reality. â€Å"While inhabiting the body of their avatar, human drivers are able to manipulate the world around them, free from the constraints of their earthly life. Pandora is a place where interspecies communication and relationships are possible.It is also a place w here a paraplegic war torn veteran can experience once again the sensations of his limbs and the joy of living. †? In the â€Å"Pandora† of social networks, a person who has a nervous personality can feel fully comfortable communicating to another person without actually being in front of them. Again Rosenfield quotes director of â€Å"Avatar† James Cameron on his experience of a submarine expedition to the Titanic, â€Å"â€Å"I'm operating it, but my mind is in the vehicle. I felt like I was physically present inside the shipwreck of Titanic. So, it was this absolutely remarkable experience. †? As technology advances, we can expect to see more of these virtual realities develop. It’s inevitable that we will be communicating with one another more and more through online networks than by person anymore. The line between reality and hyper reality will become 7. Rosenfeld N, Kimberly (Terminator to Avatar: A Post Modern Shift, 2010) 8. Rosenfeld N, K imberly (Terminator to Avatar: A Post Modern Shift, 2010) blurred for post-modern society as more people use online identities to interact.The way we used to express ourselves, from the way that we dress or the way that we talk, will be another fad lost in history. Technology will use a search engine to influence our decision on which person we want meet and communicate with. Profiles and â€Å"Avatars† will replace human personalities and online worlds will become to feel much easier to live in. Rosenfeld talks about how the movie Avatar is a prediction on how society is slowly changing their views and attitudes toward the â€Å"real life†. â€Å"We find in Pandora simulacra of a post humanist future.The utopian quality of this film’s magical world has even resulted in an uncommon public reaction, post viewing depression, as several news sources have reported. Maybe the public is especially sensitive to the contrast between the film’s hyper reality and the current reality of our depressed economy. When these viewers walk out of the theater, they are let down at returning to live in the actual world they must inhabit†. ? 9. Rosenfeld N, Kimberly (Terminator to Avatar: A Post Modern Shift, 2010) Works Cited 1. Statistic Brain â€Å"Online Dating Statistics†, online http://www. tatisticbrain. com/online-dating-statistics (6/20/2012) 2. Ward, Glenn â€Å"Teach Yourself† (Teach Yourself, 1998) Wikipedia, â€Å"Christopher Dorner† online http://en. wikipedia. org/wiki/Christopher_Dorner (2/13/2013) 3. Oregon Business Report, â€Å"45% Employers use Facebook-Twitter to screen job candidates† online http://oregonbusinessreport. com/2009/08/45-employers-use-facebook-twitter-to-screen-job-candidates (8/24/2009) 4. Rosenfeld N, Kimberly (Terminator to Avatar: A Post Modern Shift, 2010) 5. Satrapi, Marjane â€Å"Persopolis† (Random House Inc, 1969)

School Shooting Essay - 732 Words

Research in school shootings have been administered in many categories, including sociology, psychology, and etc. past studies, and direct later studies in school shootings, offers a sociology stand point for understanding the differences of school shooting incidents, including rampage shootings, mass murders shootings, and examining the mass media dynamic of school shootings; as well as presenting a combination of causes said in the research, including those on the individual, community, and social levels. Suggestions for studies in the future in school shootings are still yet to be explored. In school-related shootings, the public has been seen to dramatize the nature of the crime, induce strong public exclamation, and with good reason.†¦show more content†¦School shootings were an ascendant social problem, often because the events gathered public interest, which contributed to the perception that school shootings were a new form of violence occurring with increased frequency and intensity. An initial requirement is to define the phenomenon of school shootings and the subcategories of incidents, the varying motives and identities of the perpetrators of school shootings. While rampage attacks are a difference in school shooting incidents that have captured the sculpted eye of mass medias attention in the last decade, a broader historical perspective reveals a wide range of school-related shooting incidents. the school-related mass murder incident, typically carried out by an individual who targets categories of individuals or the school institution in general. School-related mass murders are a branch of mass murder incidents, where an adult perpetrator, who is not a current or former student or employee, targets a school institution or group of students, selected for their importance. As in rampage cases, such incidents are sometimes labeled postal-type shootings, and are typically people who desire power. Mass medias vital role in the public perception of school shoot ings is seen as a social problem, and social scientistsShow MoreRelatedSchool Shootings in America Essay1808 Words   |  8 PagesThe school shootings at Westside Middle School were orchestrated by two juveniles. On Monday, March 30, 1998 two boys ambushed students and teachers outside Westside Middle School in Jonesboro, Arkansas. Andrew Golden a youth of 11 years and Mitchell Johnson who was 13 years old were responsible for this hideous tragedy. Apparently, Mitchell Johnson hid in the woods while Andrew set off the fire alarm causing the students and teachers to run out of the building. Armed with three stolen rifles andRead MoreThe Parents Role in School Shootings Essay1196 Words   |  5 PagesWhen children commit a horrible act such as a school shooting their parents often look for someone or something to blame rather than looking at what role they, as parents, may have had in the tragedy. The often targeted entertainers, video game developers, teachers, drug companies, and writers are rarely, if ever, responsible for such tragic outcomes and, unfortunately, often become victims as a result of lawsuits filed in an attempt to place blame on them. The parents of dangerous children mustRead MoreMass School Shootings in America Essay1334 Words   |  6 PagesAmerican schools became dangerous places at the end of the twentieth century. Children as young as twelve and thirteen came to school not to study but to shoot as many people as possible. Even as these students transformed schools into war zones, teachers and other students did what they could to restore order and to save lives. In doing so, they became unlikely heroes on America’s latest battlefield. Although violence in schools is nothing new, multiple shootings are. According to the 1999 AnnualRead MoreMass School Shootings in America Essay499 Words   |  2 Pagesnever happen in my school.† This is one of the first thoughts that goes through a students mind when they hear about a school shooting. The fact is though, it can. School shootings can happen at any school at any time. Lack of security is only a small part of the problem. The major issue lies in the low morality of students and warning signs overlooked by administration. nbsp;nbsp;nbsp;nbsp;nbsp;Not all, but a majority of the students are cold-blooded killers. And shooting fellow students isRead More We Must Work to Prevent Mass School Shootings Essay1394 Words   |  6 Pages There have been many horror stories in the news about mass shootings at schools. The public, and even the president of the United States, is asking if anything can be done to prevent these tragedies. There are many theories on why students kill their peers at schools; these range from increased violence in video games and movies to bullying troubles at school. Almost always, the perpetrator suffers from some form of mental illness (Khadaroo). Because of this, motives for these crimes areRead MoreEssay On School Shootings1066 Words   |  5 Pages WHAT ARE SCHOOL RAMPAGE SHOOTINGS School rampage shootings, also referred to as mass school shootings, involve one or a small number of perpetrators whose goal is to inflict as much harm as possible on as many people as possible within a school setting. It is a violent act. There is a distinction made between violence and aggression on the continuum of severity . As noted by Bushman and Huesmann (2010), while aggression is an intent to harm another person who does not want to be harmed, violenceRead MoreArgumentative Essay On School Shootings898 Words   |  4 PagesThere has been an average of one school shooting every week in America since the Sandy Hook shooting. On the fourteenth of December, 2012, in Newtown, Connecticut, 20-year-old Adam Lanza not only killed his mother in her home, but also twenty children and six members of staff at the Sandy Hook Elementary School. This was to be the third deadliest mass shooting in U.S. history. The Huffington Post reports that as of 2014, there have been over 200 school s hootings. These have resulted in at least 94Read More School Shootings in America Essays5717 Words   |  23 PagesConnecticut, Georgia, Colorado, Virginia, Oregon, Michigan, and Tennessee are the sites in which some of the most viscous school crimes have occurred. In this day and age it seems as if school isnt a safe haven for Americas children anymore. School shootings are on the rise more than ever in todays society with kids as young as 9 years old committing these gruesome crimes against their classmates and instructors. To see this type of action among kids is heartbreaking and sad. People wonderRead MoreSchool Shootings in America Essay2422 Words   |  10 Pagesdead and 23 wounded in a High School, Four girls and a teacher are shot to death and 10 people wounded during a false alarm in school†¦Those were the headlines of all newspapers at one time or another. High School shootings have been occurring throughout the United States. Why is it that a student would come into their school and open fire? Why is it that no one notices the signs before the incident? How is it possible that they were able to bring the firearm into the school? Those are some questionsRead MoreMedia Studies : Video Games1360 Words   |  6 PagesMedia Studies Essay - AS91254 Violence in Video Games In recent years there have been plenty of cases of music, people, games, etc, being used as scapegoats so authorities can jump to conclusions about violent events and blame these as the main reason for such an event to take place. To give people an answer that may not even have any supporting evidence. Nowadays violence in video games is up to the level of killing people as the norm with many popular titles having this apart of the game. In